Biofilm is formed when bacteria develop complex communities. These communities can mature if they are not disrupted into more destructive bacteria. Biofilm exposed to excess carbohydrates produces a Gram positive carbohydrate fermenting bacteria. These bacteria contribute to demineralization and ultimately caries. Gram negative anaerobic bacteria dominated biofilm accumulated sub and supragingivally contribute to gingivitis and periodontitis Disruption may be achieved mechanically and chemically. Mechanical disruption is performed by physically removing plaque by brushing or flossing. Chemical disruption is achieved with oral rinses.